Physical Specifications:
| Size | 1kg |
| Molecular formula | C4H11NO3 |
| Molecular weight | 121.14 |
| Purity | >99.8% |
| Lead | <0.0001% |
| Moisture | <1.0% |
| Iron | <0.0001% |
| Insolubles | <0.005% |
| Copper | <0.0001% |
| A280 (1M, water) | <0.05 |
| Magnesium | <0.0001% |
| Arsenic | <0.0005% |
Molecular Biology Specifications:
| DNase assay: | None Detected |
| RNase assay: | None Detected |
| Protease assay: | None Detected |
Recommended Use:
IBI Molecular Biology Grade TRIS is commonly used in biological and electrophoresis buffer systems. Located in the table below are some of the commonly used electrophoresis buffers and their recipe as a concentrated stock.
Stock Solutions:
| Buffer | Concentrated stock solution (per liter) |
| TRIS-acetate 50X: | 242 g TRIS base |
| (TAE buffer) | 57.1 mL glacial acetic acid |
| | 100 mL 0.5 M EDTA (pH 8.0) |
| TRIS-borate 5X: | 54 g TRIS base |
| (TBE buffer) | 27.5 g boric acid |
| | 20 mL 0.5 m EDTA (pH 8.0) |
| TRIS-phosphate 10X: 1 | .08 g TRIS base |
| (TPE buffer) | 15.5 mL 85% phosphoric acid |
| | (1.679 g/ mL) |
| | 40 mL 0.5 M EDTA (pH 8.0) |
| TRIS-glycine 5X: | 15.1 g TRIS base |
| | 94 g glycine (pH 8.3) |
| | 50 mL 10% SDS |
Storage: Store at room temperature and protected from moisture.
